Thursday, June 13, 2013

Species Priority List

The list of approxiamtely 200 priority species for The Barcode of Life Project- South Africa was decided on last week at a meeting attended by various role players from UJ, SANBI and CBOL.

Plants represent the largest section of species of to be barcoded, with invertebrates and marine species coming in second. The remaining species on the list consist of representatives from fish, reptile, birds and mammals.

The selection was roughly based on the TOPS list, but further narrowed down during meetings by only selecting species that are threatened by trade, and not by other factors such as habitat destruction.

The list also focused specifically on species that are traded as parts and derivatives, which may be difficult to identify. For instance, a leopard pelt is easily and readily identifiable. However, certain species of beetles are not so easy to identify as adults, and then we have not even started looking at them in their larvae stage. Also certain plants, such as cycads, are stripped of their leaves during transport which makes identification much more of a challenge.

Animal and plant derivatives or powders, as are often found in South African traditional medicines, or muthi, also present an obstacle for taxonomists and can only be identified by DNA analysis.

Although not exhaustive, this list can be added to or revised at a later date as resources increase. For now, approximately 200 priority species have been selected for immediate DNA barcoding to assist in trials and prosecution of wildlife crimes in a court of law.





Wednesday, June 5, 2013

Workshop on Legal Standards for DNA evidence from wildlife in South Africa

Officials from all spheres of conservation, legislation, prosecution, science and law enforcement gathered today at the Pretoria National Botanical Gardens to attend the Workshop on Legal Standards for use of DNA evidence from Wildlife in South Africa.

The BoWP aims to demonstrate the value of DNA barcoding in the investigation and prosecution of wildlife, conservation and biodiversity crime. The purpose of the workshop was to bring together the various partners  and role players together to establish and understanding of the project's goals and structure;  reviewing the procedures for establishing the admissibility of new types of scientific data as evidence in South African courts and the standards for such evidence; and agreeing on a plan of action and a timetable for establishing the admissibility of DNA barcode evidence.

The welcome and introduction of various participants was facilitated by Michelle Hamer of SANBI, after which David Schindel of CBOL gave a brief overview of DNA barcoding, as well as the components involved in the legal standard for admissibility. Discussion was encouraged about a variety of topics relating to this, including the underlying scientific credibility of DNA barcoding and method validation studies, characteristics of the reference sequence database and reference specimens, procedures used in the construction of the database, the collection and handling of evidence, laboratory procedures used on evidence, data analysis using the reference sequence database, the accreditation, certification and training of laboratory staff and expert witnesses and the importance of geographical and population data in investigation and prosecution.

The interest in and importance of the project was evident during a brief coffee break, where participants continued to engage in discussions and debates, ranging from scientific discussions about taxonomy, zoology and botany to current issues such as rhino poaching, conservation policies and legislation and ivory trade.

One of the main topics of discussion when the workshop resumed was the necessity of the chain of custody and evidence collection and handling. One of the points that was clearly made was that lawyers and scientists think differently! As Dania Bruwer from the National Prosecuting Authority stated when discussing the chain of evidence, it is rarely the scientific data that is attacked in court, but rather the human process of collection and possible contamination during analysis that is a weak spot for defense. This process should not only include the suspect sample collected on the crime scene, but may also need to include the sample specimens collected for the reference database. The main obstacle seems to be collaborating the methods and expectations and finding a middle ground between the scientific standards and the legal standards. The need for secure storage and separating academic and forensic laboratories was emphasises as a procedural difference that needs to be addressed.  

The workshop was a massive success, with many key points identified that will need to be addressed as the project progresses in the future.

Tuesday, June 4, 2013

THE RACE IS ON : Why is DNA barcoding important?

THE RACE IS ON: WHICH COUNTRY WILL BE THE FIRST TO SUCCESSFULLY USE DNA BARCODING EVIDENCE TO CONVICT WILDLIFE CRIMES IN A COURT OF LAW!


Google, famous for their internet search engine, has made a US$3 million Global Impact Award to the Smithsonian Institution in Washington, DC for a project on endangered species. South Africa is one of six countries selected as partners in the project. The others are Kenya, Nigeria, Mexico, Brazil and a Southeast Asian country to be selected soon. The Barcode of Wildlife project will take DNA barcoding to the next level in terms of real-world practical applications were we will test the effectiveness of DNA barcode evidence in investigation and prosecution of wildlife crime by combining scientific research and law enforcement to protect endangered species. This is an effort to assist border and law enforcement officials, park rangers and other regulatory officials in the detection of illegal trafficking of protected species, which is currently ranked amongs the most lucrative industries in the world, slightly less than illegal drugs, human trafficking and arms dealing. The identification of species is generally work for the taxonomists, but this is not possible at every border post, and in many cases the specimens have crossed the border before an identification could be made by a professional. If border officials, for example, confiscate a box of white powder - is it ground up rhino horn, lion bone, an endangered plant root or something more innocent? If a piece of red meat is presented - does it come from a cow, or from a primate? Identification can be difficult as traffickers are taking more drastic measure to cover their tracks. These questions, and many others like them, can only be answered by using DNA technologies such as DNA barcoding.

DNA barcoding was proposed in 2003 as a way to identify species rapidly and cheaply using a short, standarised DNA sequence. According to Dr David Schindel from the Smithsonian Institute in Washington, it can be used to identify a species in the same way that the universal product code - the barcodes used on products in a supermarket - distinguish the product that you are buying from countless others on the shelf. DNA barcoding is similar in the sense that once a reference library has been created (by sequencing the barcode region of DNA) it would allow the user to quickly look up and identify the species in hand.

South Africa has been especially active in the collection of DNA material from both plants and animals for this data bank.

BWP takes this to the DNA barcoding to the next level in terms of real-world, practical applications.

The project will bring together people from all sectors to ensure that all the relevant officials have access to this technology. Meetings have already been attended by a variety of parties, including the Department of Environmental Affairs, airport customs officials, the South African Police Service, SANBI, prosecutors, as well as scientists from universities and museums to ensure that this truly is a cross-frontier operation, with all participants realising the importance of the opportunity at hand, and tackling their respective roles with passion and commitment.

The ultimate goal of the project is that towards the end of 2014 DNA barcoding evidence will be used in a court of law. That implies that within two years, somebody will go to jail for illegal poaching and trafficking, due to this unique and groundbreaking research and technology.

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